Chemostat Culture for Yeast Physiology.
نویسندگان
چکیده
The use of chemostat culture facilitates the careful comparison of different yeast strains growing in well-defined conditions. Variations in physiology can be measured by examining gene expression, metabolite levels, protein content, and cell morphology. In this protocol, we show how a combination of sample types can be collected during harvest from a single 20-mL chemostat in a ministat array, with special attention to coordinating the handling of the most time-sensitive sample types.
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ورودعنوان ژورنال:
- Cold Spring Harbor protocols
دوره 2017 7 شماره
صفحات -
تاریخ انتشار 2017